The crash happened at 1.15pm local time today when the bus plunged into a ravine on the GM-2 highway in La Gomera.
All of the passengers, 24 adults and three children, were British.
The person killed in the bus crash was a British man, the Foreign Office has said. He was aged 77, it is understood.
Four people are seriously injured, emergency services in the Canary Islands said in an update.
"Emergency health services attended to the 28 occupants of the bus, 27 tourists of British nationality and the driver," local officials said.
Foreign secretary Yvette Cooper said in a post on X: "My thoughts are with those affected by the tragic incident involving a bus carrying British holidaymakers in the Canary Islands."
She said the government was in touch with the local authorities and ready to support the Britons and their families.
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Two of the most seriously injured, a 73-year-old man and a 42-year-old man, were airlifted to hospitals on the larger neighbouring Canary Island of Tenerife.
Two people with serious injuries were being treated at Nuestra Senora de Guadalupe Hospital in San Sebastian de La Gomera on the island where the crash took place. Another 23 with minor injuries were also taken there.
Images shared by the Canary Islands government on social media appear to show the bus had come off the road at a hairpin bend.
The GM-2 road where the incident took place is high up, with spectacular views out to sea. La Gomera, the second smallest of the eight Canary Islands, is marked by the steep terrain of volcanic mountains, dense forest and cliffside villages.
A statement from the British embassy in the Spanish capital, Madrid said: "Our thoughts go out to those affected by this tragic incident.
"We are aware of the situation, and we stand ready to support British nationals.
"We are also in touch with local authorities on the ground."
A Foreign Office spokesperson said: "We are supporting the family of a British man who died in the bus accident in the Canary Islands and are in contact with the local authorities."
Sentebale was founded by Harry in 2006 to help young people with HIV and AIDS in Lesotho and Botswana, but he resigned as a patron in March 2025 after a public falling-out with the chair of the board, Sophie Chandauka.
According to a record made public on Friday, Sentebale lodged a defamation claim last month at the High Court against Harry and Mark Dyer, who was also a trustee of the charity.
No documents are currently available in the claim, which was originally filed on 24 March.
A spokesperson for Prince Harry and Mr Dyer said the pair "categorically reject these offensive and damaging claims", after the legal action was launched.
Sentebale said it lodged a defamation claim following what it called a "co-ordinated adverse media campaign" which it said had damaged its reputation.
In a statement, its board of trustees and executive director said: "The charity seeks the court's intervention, protection and restitution following a co-ordinated adverse media campaign conducted since March 25 2025 that has caused operational disruption and reputational harm to the charity, its leadership and its strategic partners.
"The proceedings have been brought against Prince Harry and Mark Dyer, identified through evidence as the architects of that adverse media campaign, which has had significant viral impact and triggered an onslaught of cyber-bullying directed at the charity and its leadership.
"Sentebale has experienced the adverse media campaign as false narratives circulated through the media about the charity and its leadership, attempts to undermine its relationships with staff, existing and prospective partners, and the forced diversion of leadership time and resources into managing a reputational crisis not of the charity's making."
Harry co-founded the charity, alongside Prince Seeiso of Lesotho, which was aimed at enhancing the lives of vulnerable children in the southern African country.
The pair both stepped down last year in a dispute with chairperson Dr Chandauka.
The initial rift emerged in 2023 after trustees began plans for a new fundraising strategy in the US.
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The "serious dispute" was exacerbated by strategic and financial difficulties for the charity following the COVID-19 pandemic, according to a report from The Charity Commission (CC).
The CC criticised both sides for allowing the dispute to be played out in public.
Commission CEO David Holdsworth said they enabled the rift to "harm the charity's reputation", which in turn jeopardised its "ability to deliver for the very beneficiaries it was created to serve".
He was first arrested on Saturday, 4 April, and released on bail. He was later re-arrested on Thursday, 9 April, the Metropolitan Police said.
Officers were called to the scene in Lord Warwick Street, which is close to the Woolwich Ferry and Woolwich Dockyard train station, to reports of a shooting at around 3.40pm on 2 April.
Eghosa was pronounced dead at the scene.
The 16-year-old, from Romford, will appear at Thames Magistrates' Court on Saturday.
Eghosa's family continue to be supported by specialist officers, the force added, with investigations ongoing.
The 16-year-old was first arrested, along with a 19-year-old man, on suspicion of murder. A 46-year-old man was also arrested on suspicion of assisting an offender.
All three were subsequently released on bail, police said.
The day before, a 14-year-old boy, 16-year-old boy and 18-year-old man were arrested in connection with Eghosa's death.
They have also been bailed while inquiries continue.
On 10 April, a further 16-year-old boy was also arrested on suspicion of murder and remains in custody.
Under the terms of the deal, Britain would cede sovereignty of the archipelago to Mauritius, but continue to lease the largest island, Diego Garcia - which hosts a joint US-UK military base.
Now the bill underpinning the deal has been pulled from the upcoming King's Speech, where the government's agenda for the coming parliament is revealed, it is understood.
The US president initially supported the agreement, but changed course in January and branded it "an act of great stupidity" during a dispute with his NATO allies over Greenland.
The British government stands by the deal and will attempt to persuade Trump to change his mind but has acknowledged that it cannot proceed without his backing, The Times has reported.
A government spokesperson said: "Diego Garcia is a key strategic military asset for both the UK and the US.
"Ensuring its long-term operational security is and will continue to be our priority - it is the entire reason for the deal.
"We continue to believe the agreement is the best way to protect the long-term future of the base, but we have always said we would only proceed with the deal if it has US support.
"We are continuing to engage with the US and Mauritius."
It comes after Mr Trump repeatedly criticised Prime Minister Keir Starmer for its stance on the war in Iran.
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The Conservative leader of the Opposition, Kemi Badenoch, branded the deal a "surrender" and said its "rightful place" was on the "ash heap of history".
In a post on X, formerly Twitter, she wrote: "That it took so long is another damning indictment of a prime minister, who fought to hand over British sovereign territory and pay £35 billion to use a crucial military base which was already ours."
Air Chief Marshal Sir Richard Knighton said the updated iteration of what was called the Government War Book would require people to think differently about resilience, drawing on lessons from the Cold War but "in a modern context, with a modern society, with modern infrastructure".
In an interview on Friday at the London Defence Conference, he also revealed that a threat by the UK to seize ships that are part of Russia's murky "shadow fleet" is already having an effect, even though British forces have yet to board any vessels.
The chief of the defence staff said the mere knowledge that London was ready to target a sanctioned tanker was forcing Moscow to escort them or divert them away from UK waters.
However, shadow fleet ships have still been spotted off the coastline without being stopped.
Asked whether an actual boarding operation was imminent, Air Chief Marshal Knighton said: "Be in no doubt. We are ready."
Perhaps the biggest challenge for the military chief is a push to put the Royal Navy, army and Royal Air Force back on a war footing after decades of underfunding under previous Conservative and Labour governments since the collapse of the Soviet Union.
Sir Keir Starmer and John Healey, his defence secretary, have promised to lift defence spending to 3.5% of GDP from just over 2% - but not until 2035.
They are also yet to release a crucial 10-year investment plan for the armed forces - which sets out what weapons and capabilities the Ministry of Defence (MoD) will procure.
The Defence Investment Plan should have been published last autumn - but Mr Healey, in a separate Sky News interview, would not even say whether it would be out by this summer.
The delay means much of the UK's defence industry is in limbo, awaiting the promise of new cash to become a reality.
Air Chief Marshal Knighton signalled that the hold up was because the MoD was holding out for more money to be made available from the Treasury faster.
"What I want is a defence investment plan that is properly funded and delivers what we want," he said.
"If that takes a bit longer, I'd rather have something that works and we can deliver."
A key revelation in his comments was about the plan, led by the Cabinet Office and involving all other government departments, to produce a new version of the old war book.
Conceived during the First World War, the government's previous collection of top secret, regularly rehearsed and updated war books made the UK one of the best prepared nations in the world for conflict - and one of the most resilient.
A 1976 copy - a large bundle of hand-typed pages, bound together by string - contained detailed lists and signposted the way to complementary plans about how to mobilise not just the military but also civilians and industry in a crisis, as well as shutting schools, clearing hospitals, rationing food, and even storing national treasures.
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That all changed after the Cold War ended and by the early 2000s, the entire UK war book system, which cost a lot to maintain, was quietly shelved.
Asked whether Britain was reviving the old government war book, Air Chief Marshal Knighton said: "I think that's right."
He set out what that would look like.
"NATO describes the transition to conflict as a military component, but it also has a civilian component," the defence chief said.
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This includes ensuring critical national infrastructure, such as power stations and water supply, are resilient not just to natural disasters such as floods but also to the threat of war.
"I talked before Christmas of the need that when we think about renewing our water infrastructure or electricity or transport infrastructure, to think about the threat of action from an adversary that is above the threshold of war, not just a hybrid threat," he said.
"And think about how we build in that resilience as we renew it and that requires making some different choices and different priorities and that work that the Cabinet Office is doing across the whole of government is something that I really welcome."
He said civilians need to be aware that the relative peace the UK has enjoyed over the past 30 years is under increasing threat.
"That requires us to educate ourselves and help the population understand some of those threats and help them understand what they can do to support the nation and potentially support the armed forces."
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